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timp3 expression  (OriGene)


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    OriGene timp3 expression
    Timp3 Expression, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/timp3 expression/product/OriGene
    Average 94 stars, based on 2 article reviews
    timp3 expression - by Bioz Stars, 2026-03
    94/100 stars

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    Sequences of primers used in real-time PCR.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: Sequences of primers used in real-time PCR.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques:

    IL-6 inhibits TIMP3 expression through STAT3 activation and TIMP3 is upregulated in the cisplatin sensitive group. (A) Western blotting analysis of TIMP3, p-STAT3, and STAT3 protein levels in extracts from Saos2 and Saos2-lung cells treated with 20 ng/mL IL-6 for 24 h. (B,C) The results are expressed as the ratio of TIMP3 to GAPDH, and p-STAT3 to STAT3. (D) The expression of TIMP3 was measured in Saos2 and Saos2-lung cells with or without IL-6 treatment via real-time PCR, with GAPDH as a housekeeping gene. (E) The expression of TIMP3 was measured in Saos2 and Saos2-lung cells via western blotting, with GAPDH as an internal reference. (F) Sub-confluent Saos2 and Saos2-lung cells were grown in serum-free DMEM for 24 h, and the IL-6 level in the supernatant was detected using an ELISA. (G) Immunohistochemical analysis of TIMP3 was carried out in the tumor samples from different patients. Data are presented as the means ± SD. ∗∗ P < 0.01, ∗ P < 0.05. All data were obtained from at least three independent experiments.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: IL-6 inhibits TIMP3 expression through STAT3 activation and TIMP3 is upregulated in the cisplatin sensitive group. (A) Western blotting analysis of TIMP3, p-STAT3, and STAT3 protein levels in extracts from Saos2 and Saos2-lung cells treated with 20 ng/mL IL-6 for 24 h. (B,C) The results are expressed as the ratio of TIMP3 to GAPDH, and p-STAT3 to STAT3. (D) The expression of TIMP3 was measured in Saos2 and Saos2-lung cells with or without IL-6 treatment via real-time PCR, with GAPDH as a housekeeping gene. (E) The expression of TIMP3 was measured in Saos2 and Saos2-lung cells via western blotting, with GAPDH as an internal reference. (F) Sub-confluent Saos2 and Saos2-lung cells were grown in serum-free DMEM for 24 h, and the IL-6 level in the supernatant was detected using an ELISA. (G) Immunohistochemical analysis of TIMP3 was carried out in the tumor samples from different patients. Data are presented as the means ± SD. ∗∗ P < 0.01, ∗ P < 0.05. All data were obtained from at least three independent experiments.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Expressing, Activation Assay, Western Blot, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining

    IL-6 and p-STAT3 expression after the overexpression or knockdown of TIMP3 in Saos2-lung cells. (A) The identification of TIMP3 overexpression and knockdown in Saos2-lung cells. (B) The results are expressed as the ratio of the expression of the target gene to that of GAPDH . (C) The expression levels of p-STAT3 and STAT3 were measured in transfected-Saos2-lung cells treated with or without cisplatin via western blotting, with GAPDH was an internal reference. The results are expressed as the ratio of p-STAT3 to STAT3. (D) IL6 gene expression in transfected-Saos2-lung cells without cisplatin treatment. (E) IL6 gene expression in transfected-Saos2-lung cells with cisplatin treatment. (F) The expression level of TIMP3, p-STAT3, and STAT3 was measured in STAT3 knockdown Saos2-lung cells with or without IL-6. Data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01. All data were obtained from at least three independent experiments.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: IL-6 and p-STAT3 expression after the overexpression or knockdown of TIMP3 in Saos2-lung cells. (A) The identification of TIMP3 overexpression and knockdown in Saos2-lung cells. (B) The results are expressed as the ratio of the expression of the target gene to that of GAPDH . (C) The expression levels of p-STAT3 and STAT3 were measured in transfected-Saos2-lung cells treated with or without cisplatin via western blotting, with GAPDH was an internal reference. The results are expressed as the ratio of p-STAT3 to STAT3. (D) IL6 gene expression in transfected-Saos2-lung cells without cisplatin treatment. (E) IL6 gene expression in transfected-Saos2-lung cells with cisplatin treatment. (F) The expression level of TIMP3, p-STAT3, and STAT3 was measured in STAT3 knockdown Saos2-lung cells with or without IL-6. Data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01. All data were obtained from at least three independent experiments.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Expressing, Over Expression, Knockdown, Transfection, Western Blot, Gene Expression

    Cell proliferation and cisplatin sensitivity after the overexpression or knockdown of TIMP3 in Saos2-lung cells. (A–D) Cisplatin sensitivity of transfected-Saos2-lung cells at 24, 48, 72, and 96 h. (E) Cell proliferation in transfected-Saos2-lung cells without cisplatin. (F) Flow cytometry assessment of apoptosis in transfected-Saos2-lung cells without or with cisplatin treatment. (G) Apoptosis rates of transfected-Saos2-lung cells with or without cisplatin treatment. (H) Hoechst staining of transfected-Saos2-lung after cisplatin treatment for 24 h. (I) The quantification of hoechst staining. The results are expressed as the ratio of p-Akt to t-Akt. Data are presented as the means ± SD. ∗ P < 0.05 between the Saos2-lung and siRNA groups, # P < 0.05 between the Saos2-scramble and Saos2-lung-TIMP3 groups, separately, in a cell proliferation assay. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. All data were obtained from at least three independent experiments.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: Cell proliferation and cisplatin sensitivity after the overexpression or knockdown of TIMP3 in Saos2-lung cells. (A–D) Cisplatin sensitivity of transfected-Saos2-lung cells at 24, 48, 72, and 96 h. (E) Cell proliferation in transfected-Saos2-lung cells without cisplatin. (F) Flow cytometry assessment of apoptosis in transfected-Saos2-lung cells without or with cisplatin treatment. (G) Apoptosis rates of transfected-Saos2-lung cells with or without cisplatin treatment. (H) Hoechst staining of transfected-Saos2-lung after cisplatin treatment for 24 h. (I) The quantification of hoechst staining. The results are expressed as the ratio of p-Akt to t-Akt. Data are presented as the means ± SD. ∗ P < 0.05 between the Saos2-lung and siRNA groups, # P < 0.05 between the Saos2-scramble and Saos2-lung-TIMP3 groups, separately, in a cell proliferation assay. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. All data were obtained from at least three independent experiments.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Over Expression, Knockdown, Transfection, Flow Cytometry, Staining, Proliferation Assay

    Apoptosis-related gene expression in Saos2-lung cells following overexpression or knockdown of TIMP3. (A) The expression levels of Bcl-2, Bax, p-Akt, cleaved caspase-3, and cleaved caspase-9 were measured using western blotting in transfected-Saos2-lung cells, with or without cisplatin. (B,C) The results are expressed as the ratio of Bcl-2 to GAPDH, Bax to GAPDH, p-Akt to t-AKT, cleaved caspase-3 to caspase-3, and cleaved caspase-9 to caspase-9. Data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. All data were obtained from at least three independent experiments.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: Apoptosis-related gene expression in Saos2-lung cells following overexpression or knockdown of TIMP3. (A) The expression levels of Bcl-2, Bax, p-Akt, cleaved caspase-3, and cleaved caspase-9 were measured using western blotting in transfected-Saos2-lung cells, with or without cisplatin. (B,C) The results are expressed as the ratio of Bcl-2 to GAPDH, Bax to GAPDH, p-Akt to t-AKT, cleaved caspase-3 to caspase-3, and cleaved caspase-9 to caspase-9. Data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. All data were obtained from at least three independent experiments.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Gene Expression, Over Expression, Knockdown, Expressing, Western Blot, Transfection

    Cisplatin sensitivity of Saos2-lung cells after TIMP3 overexpression in vivo . (A–C) Cisplatin or saline was used to treat nude mice bearing osteosarcoma cells. Saos2-lung cells with and without TIMP3 overexpression were labeled with luciferase and implanted in the tibia. The tumor volume was evaluated via in vivo bioluminescence assay at 1–5 weeks after cisplatin or saline treatment. (D,E) The mice were sacrificed, and the tumor volume was measured and calculated during the 5th week after treatment. The data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. Each group contained five animals.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: Cisplatin sensitivity of Saos2-lung cells after TIMP3 overexpression in vivo . (A–C) Cisplatin or saline was used to treat nude mice bearing osteosarcoma cells. Saos2-lung cells with and without TIMP3 overexpression were labeled with luciferase and implanted in the tibia. The tumor volume was evaluated via in vivo bioluminescence assay at 1–5 weeks after cisplatin or saline treatment. (D,E) The mice were sacrificed, and the tumor volume was measured and calculated during the 5th week after treatment. The data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. Each group contained five animals.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Over Expression, In Vivo, Saline, Labeling, Luciferase, ATP Bioluminescent Assay

    Immunohistochemical analysis of tumor samples with and without TIMP3 overexpression. (A) The expression profiles of TIMP3, cleaved caspase-3, p-Akt, PCNA, and IL-6 were evaluated using immunohistochemistry. (B,C) Quantification of positive staining was performed using Image-Pro Plus 6.0 software. The results are expressed in terms of the mean density of positive staining. (D) Apoptotic cells were detected by TUNEL staining in tissue sections. (E) Semi-quantitative data from the analysis of apoptotic cells. The data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. Each group contained five animals.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: Immunohistochemical analysis of tumor samples with and without TIMP3 overexpression. (A) The expression profiles of TIMP3, cleaved caspase-3, p-Akt, PCNA, and IL-6 were evaluated using immunohistochemistry. (B,C) Quantification of positive staining was performed using Image-Pro Plus 6.0 software. The results are expressed in terms of the mean density of positive staining. (D) Apoptotic cells were detected by TUNEL staining in tissue sections. (E) Semi-quantitative data from the analysis of apoptotic cells. The data are presented as the means ± SD. ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. Each group contained five animals.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Immunohistochemical staining, Over Expression, Expressing, Immunohistochemistry, Staining, Software, TUNEL Assay

    A schematic diagram of the proposed mechanism of cisplatin sensitivity enhancement by TIMP3 overexpression. According to the results of the current study, IL-6 inhibited TIMP3 expression through STAT3 activation, whereas conversely; TIMP3 suppressed the production of IL-6. TIMP3 also facilitates the expression of cleaved caspase-3, while inhibiting the production of p-Akt, which results in the inhibition of proliferation and the overall potentiation of apoptosis. Taken together, these events contribute to enhancing the sensitivity of osteosarcoma to cisplatin.

    Journal: Frontiers in Genetics

    Article Title: TIMP3 Overexpression Improves the Sensitivity of Osteosarcoma to Cisplatin by Reducing IL-6 Production

    doi: 10.3389/fgene.2018.00135

    Figure Lengend Snippet: A schematic diagram of the proposed mechanism of cisplatin sensitivity enhancement by TIMP3 overexpression. According to the results of the current study, IL-6 inhibited TIMP3 expression through STAT3 activation, whereas conversely; TIMP3 suppressed the production of IL-6. TIMP3 also facilitates the expression of cleaved caspase-3, while inhibiting the production of p-Akt, which results in the inhibition of proliferation and the overall potentiation of apoptosis. Taken together, these events contribute to enhancing the sensitivity of osteosarcoma to cisplatin.

    Article Snippet: For TIMP3 overexpression, Saos2-lung, MG63, and U2OS cells were transfected with green fluorescent protein (GFP)-labeled-lentiviral vectors (GeneChem, Shanghai, China) expressing TIMP3 or a vector with a scrambled control sequence.

    Techniques: Over Expression, Expressing, Activation Assay, Inhibition